Human follicles culture data
Table of Contents
Description
These data comes from a former student of this PhD course. She conducted an experiment to compare different conditions to grow pre-antral human follicles in vitro. The main outcomes were follicles grotwh and survival at 8 days.
Human pre-antral follicle were isolated from ovarian medulla donated by 14 patients undergoing unilateral oophorectomy for ovarian tissue cryopreservation. Follicles were encapsulated in 0.5% alginate and cultured for 8 days in media supplemented with 5% fetal bovine serum (FBS) (n = 171), 2.5% human serum albumin (HSA) (n = 159), 5% HPL (n = 223) or 5% UCP (n = 171).
Variables
| Variable | Explanation (Unit) |
|---|---|
| Disease | Disease of the donor |
| Age | Age of the donor |
| Patient | Donor ID |
| Treatment | media supplement (i.e., conditions studied: FBS, HSA, HPL or UCP) |
| Transport | "yes" if the tissues have been transported from local hospitals, "no" otherwise |
| Day0 | initial follicle diameter (i.e., at "Day 0", when culture starts) (micrometre) |
| Day2 | follicle diameter after 2 days or NA if the follicle is dead (micrometre) |
| Day4 | follicle diameter after 4 days or NA if the follicle is dead (micrometre) |
| Day6 | follicle diameter after 6 days or NA if the follicle is dead (micrometre) |
| Day8 | follicle diameter after 8 days or NA if the follicle is dead (micrometre) |
Data download
| Format | Download |
|---|---|
| text (csv) | HFollicles.csv |
| rda (for R) | HFollicles.rda |
Load data into R
load(url("http://paulblanche.com/files/HFollicles.rda")) head(HFollicles)
References
Adrados, Cristina Subiran, et al. "Human platelet lysate improves the growth and survival of cultured human pre-antral follicles." Reproductive BioMedicine Online 47.5 (2023): 103256. https://doi.org/10.1016/j.rbmo.2023.06.011 .
Source
Supplementary material of the publication cited above.